Principle: The –CO-NH- bond (peptide) in polypeptide chain reacts with copper sulphate in an alkaline medium to give a blue colored complex. 0000005130 00000 n x��TKo�@�#���x`@�rȣU�Fj�T=D=l`1H��'ʿ��b���r�Yf�{�7��'���o/o�������%�}�c\C,D!����?
Some tubules showed regenerating epithelial cells with multilayering and mitosis, with a predominant loss of proximal tubules. The working range for the biuret assay is 5-160 mg/mL, which is adequate for some types of industrial applications but not nearly sensitive enough for most protein research needs. The blue color continues to intensify during a 30 minute room temperature incubation. Oral copper sulfate poisoning should as soon as possible be treated with gastric lavage and then instillation of potassium ferrocyanide or activated charcoal to minimize absorption. 1 0 obj So, the basic objective of this tes… Lowry’s method for protein determination results in a more prominent colour change, i.e. This is comparable to the mortality rate of 23% among 35 patients hospitalized due to, Kurisaki et al., 1988; Bhowmik et al., 2001; Naha et al., 2012, Chuttani et al., 1965; Kurisaki et al., 1988, Encyclopedia of Food Sciences and Nutrition (Second Edition), A very old woman accidentally ingested a formulation containing zinc and, Cristina Torres-Duarte, ... Gary N. Cherr, in, , we can observe the effects of lower seawater pH and, Journal of Photochemistry and Photobiology B: Biology, Twyford Rd Bishop’s Stortford CM23 3LJ UK, Porschester 12 D-84478 Waldkraiburg Germany.
[8] Allerdings ist sie zeitaufwändiger und störanfälliger. 0000059025 00000 n [7] Die Lowry-Methode ist wesentlich empfindlicher als die Biuret-Methode wegen ihrer zweiten, zusätzlichen Farbreaktion. Intravenous injection of copper sulfate resulted in acute renal failure and oliguria, without any gastrointestinal disturbance (Bhowmik et al., 2001). 0000009953 00000 n In two cases, 50 g/L and 65 g/L hemoglobin were measured on admission to the hospital, and 1.8 mg Cu/L in serum and 5.3 mg Cu/L in whole blood, respectively, were reported.
0000010905 00000 n Metal fume fever has also been reported after exposure to copper-containing fumes and fine dusts, with the first reports dating back at least to the start of the twentieth century (Hansen, 1911; Friberg and Thrysin, 1947). So hat Hartree 1972 eine Variante der Lowry-Methode vorgestellt, die u. a. die hohe Störanfälligkeit adressiert.
He was dialysed and gradually improved over 6 weeks. It has been proposed that the jaundice may be related to either hepatotoxicity or hemolysis (Chuttani et al., 1965). One particular benefit is that, unlike other methods available at that time (e.g., Bradford and Lowry assays), the BCA Protein Assay is compatible with samples that contain up to 5% surfactants (detergents). Renal insufficiency with necrosis of tubular epithelium and edema of the medulla was found in another case (Kurisaki et al., 1988). The interaction between bio molecules is crucial for knowing their function. Figure 5. Nano CuO also alleviated the inhibition of both the methods by common interfering substances such as ammonium sulfate, glucose, EDTA, SDS, Triton X-100, dithiothreitol and 2-mercaptoethanol.