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In the second step, The BCA assay has a linear concentration range between 200–1,000 µg of protein per milliliter. endstream
6.4.9 Read the absorbance of the samples at 562 nm. The Thermo Scientific ™ Pierce BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. %PDF-1.7
A protein assay can be set up to measure the concentration of the unknown protein sample (mg/ml), or it can be set up to determine the total amount of protein in the unknown protein sample (mg). endobj
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This method combines the well-known reduction of Cu +2 to Cu +1 by protein in an alkaline medium (the <>
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BCA™ Protein Assay – Reducing Agent Compatible Standard tube 125-2,000 µg/ml BCA™ Protein Assay Standard tube or microplate 20-2,000 µg/ml Enhanced tube 5-250 µg/ml Micro BCA™Protein Assay Standard tube 0.5-20 µg/ml Standard microplate 2-40 µg/ml Modified Lowry Protein Assay Standard protocol 1-1,500 µg/ml endobj
Use the standard curve to calculate the protein concentration in mg/mL.. 6.4.10 For final protein concentration, multiply by any dilution factor which was required to bring the concentration within the linear range of the assay. 12 0 obj
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The result is an extremely sensitive colorimetric protein assay in a test tube or microplate assay format. %����
In common with the Lowry assay, the biuret reaction is the first step in the reaction that takes place in the BCA assay. The Micro BCA Protein Assay Kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature (60°C, Test Tube Procedure only). <>
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The BCA protein assay was first described in 1985 to determine protein concentration with the use of bicinchoninic acid (BCA). Nevertheless, in spite that the general principle behind this methodology is known, there are still unanswered questions regarding the chemistry behind the assay and the experimental conditions commonly employed. <>
The BCA Protein Assay is a detergent compatible formulation based on Bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.