The total protein concentration is exhibited by a color change of the sample solution in proportion to protein concentration, which can then be measured using colorimetric techniques. Determination of Total Protein by the Lowry Method Using the BioTek Instruments’ ELx808 Microplate Reader It’s Fast, It’s Easy, and It Turns Blue Quantitation of total protein content of samples is a measurement common to many applications in basic science and clinical research.
The Lowry assay (1951) is an often-cited general use protein assay. Lowry adds phosphomolybdic/phosphotungstic acid also known as Folin-Ciocalteu reagent. Application of Lowry Protein Determination to Influenza Vaccine. Quantitation of total protein content is a measurement common to many applications in basic science research and routine clinical laboratory practice. The Lowry protein assay is a biochemical assay for determining the total level of protein in a solution. One of these is the Lowry protein assay, one of the most common methods used to measure the concentration of protein in a sample. Because of the tendency of the virus to aggregate, additional techniques such as sonoration were needed. Protein assay: principle and application Protein assay represents one of the most fundamental and yet important techniques of life science research. The Lowry protein test has been successfully applied to the determination of microgram amounts of protein in influenza vaccine. By continuing you agree to the use of cookies. Precision among replicates was good; the greatest difference of a single value from the average was 1.7%. This work followed earlier approaches to the application of the Van Slyke (3) method of protein precipitation and the Lowry method of protein determination to evaluation of purified virus preparations that were carried out in the Lilly Laboratories by Dr. John Ross. The writer wishes to express his gratitude for guidance and encouragement to Mr. Max M. Marsh and Dr. Harry A. Published by Elsevier Inc. All rights reserved. Copyright © 2020 Elsevier B.V. or its licensors or contributors.
It is named for the biochemist Oliver H. Lowry who developed the reagent in the 1940s. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. The specific activity of a particular enzymatic activity is of particular importance when proteins are being purified or different samples are being co… He also thanks Dr. R. S. Baker, Biological Research Department of Eli Lilly and Company, for samples of influenza vaccine. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature, cited over 300,000 times. Lowry Method The Lowry protein assay is based on the biuret reaction with additional steps and reagents to increase the sensitivity of detection. Most biochemical studies that involve the measurement of a biological activity require the normalization of that activity to the protein content.