The basis for this assay is the reduction of copper in alkali by protein (biuret reaction) in the presence of the chromogenic agent bicinchoninic acid. 7) After mixing, add 180 µL of the BCA Reagent mixture to each well of the 96 well plate. Title: Protocol for BCA Protein Assay Using a NanoDrop 2000/2000c Spectrophotometer Subject: The Thermo Scientific Pierce BCA Protein Assay combines the protein-induced biuret reaction with the highly sensitive and selective colorimetric detection of the resulting cuprous cation \(Cu1+\) by bicinchoninic acid \(BCA\). The assay can be completed in 5 minutes at room temperature- eliminating the need to wait or expose samples to elevated temperatures. The Bicinchoninic Acid (BCA) Protein Assay is suitable for quantifying protein solutions in 1ml assays or in micro-wells. The result is an extremely sensitive colorimetric protein assay in a test tube or microplate assay format. molecules of BCA with one cuprous ion. Assay Principle. The result is an extremely sensitive colorimetric protein assay in a test tube or microplate assay format. The Micro BCA™ Protein Assay Reagent Kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature (60°C). Note: All specifications and protocol instructions presented below are specific to the pedestal mode of NanoDrop One/Onec instruments. The pathlength in each assay is dependent on the assay container (cuvettes or multiwell plates) and/or the reaction volume. Pipette 25µl of each standard and prote in samples into a microplate well. the micro-volume capability of the Thermo Scientific™ NanoDrop™ Spectrophotometers, the assay provides an accurate means of protein quantitation with minimal consumption of sample. Rapid Gold BCA Protein Assay Kit protocol. 6) Prepare enough BCA assay reagents by combining 20 mL of Reagent A with 400 µL of Reagent B. Rapid Gold BCA Protein Assay Kit protocol This colorimetric assay provides the high sensitivity and linearity associated with the BCA assay, but in a fraction of the time it takes to perform a standard BCA assay. Micro BCA Protein Assay Kit is a ready-to-use, detergent-compatible, Western blot related, total protein analysis reagent, used for the quick determination of total protein concentration of dilute protein solutions by measuring absorbance at 562 nm and comparing to a protein standard absorbance vs. concentration curve. Protocol name: BCA Protein Assay.prt The protocol calls for an endpoint absorbance measurement at 562 nm. In conjunction with the micro-volume capability of a Thermo Scientific™ NanoDrop™ spectrophotometer, the assay provides an accurate For my BCA assays, I dilute the standards to final concentrations: (0, 250, 500, 1000, 1500 and 2000μg/m L) to generate a standard curve and determine an absorbance- … Please refer to the reagent The Micro Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions and is a modification of the Bicinchoninic Acid (BCA) Protein Assay for dilute protein samples (0.5 -20µg/ml). The Micro BCA Protein Assay Kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature (60°C, Test Tube Procedure only). Preparation of Standards and Working Reagent Cat.
786-570 The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples.