(1992) Modification of the BCA protein assay to eliminate lipid interference in determining lipoprotein protein content. ]�l�+.�t�T_�[w����K��)�_��oN��[-#�f|90���I[�j�u�Wg �5t���di;���m�6t9�[�T�}�ѻ� ۙ��;����APP�9�II�"�aF!%�0�����KT/���k� to�,�K�� ہ e`�c�f���j8tgpc��x���]E�F���a��r�� [�F�F�]LA�r�a3��00zr2MkV`H`�b�����TͰ�A�����c�p�CB���H!���R��"������. 0000006707 00000 n trailer << /Size 122 /Info 50 0 R /Root 63 0 R /Prev 300662 /ID[<0f672dccbbcece77ba96def5ba540a33><0f672dccbbcece77ba96def5ba540a33>] >> startxref 0 %%EOF 63 0 obj << /Type /Catalog /Pages 51 0 R /Threads 64 0 R /OpenAction [ 68 0 R /XYZ null null null ] /PageMode /UseNone /JT 61 0 R >> endobj 64 0 obj [ 65 0 R 66 0 R 67 0 R ] endobj 65 0 obj << /I << /Title (1296w)>> /F 80 0 R >> endobj 66 0 obj << /I << /Title (23225\t23227)>> /F 81 0 R >> endobj 67 0 obj << /I << /Title (Substance)>> /F 19 0 R >> endobj 120 0 obj << /S 289 /T 445 /Filter /FlateDecode /Length 121 0 R >> stream %���� Analyt. 0000103783 00000 n 7) After mixing, add 180 µL of the BCA Reagent mixture to each well of the 96 well plate. The Micro BCA Protein Assay Kit uses concentrated reagents and a protocol that utilizes an extended incubation time at an elevated temperature (60°C, Test Tube Procedure only). x��=�r�8���?�j����6M�H0�յ��;��$�M{k�� ��ecKjY�L�a�j^���\ �v�[s�H ����vws�8�e��r�[�_//�Oǧ��ߏO�n��W7���f����������T����J[�u����5ʆPR`�N�? 0000001927 00000 n 2 0 obj Add 10µl of BSA standards (stored in -80°C freezer) to each of the appropriate wells. C. TCA Concentration-BCA Assay Protocol procedure. A further advantage of the BCA assay is that it is generally more tolerant to the presence of compounds that interfere with the Lowry assay. 285 0 obj <>stream Title: Protocol for BCA Protein Assay Using a NanoDrop 2000/2000c Spectrophotometer Subject: The Thermo Scientific Pierce BCA Protein Assay combines the protein-induced biuret reaction with the highly sensitive and selective colorimetric detection of the resulting cuprous cation \(Cu1+\) by bicinchoninic acid \(BCA… (By modifying the protocol you can change the range to 20-2000 µg/ml. Add 60 µL of deionized water to the samples (dilute by a factor of 4). “Measuring Protein with BCA Assay.” The Protocol Place. ��ͧ��?���wd�ˢ?�\a�}����u>-�g�i����3�Ivŀ�墮��/�%�U��`��Y1�+p՟��§L�/�7���|�]r��=�Tt�j re��������H��F:j8���b��Av��b(�������B�~�G���Us<1���q5���џ���aH��U�%t*���U�X;�b���:YWF��2��4N�N��bH9hU�]9+V�xb��,��k�,`�����"{�Bʴ]����ݗ�����W�rU_�W�]n��J����Υ�VD9�WCD�?%V �@6S<0$)�� ІB6-}|�f=�%���|8�R�,���c(-CBzDi�Q(�Uq���(��E�Ζ��c;� 1�z���R�>���حn������:����ux* �(;����Cд7 �:|���Z�Z�v+��g�����bJU3�q0}k˂�ƥk_��B�6 ����`cR%�7�G6-j-2آ��B�,`����:�5�үw�DŽ���"8��vWaY���t4Fm���G������a�=9����kvP7J�~�*��n�ٛ���UJ������'ӈ�����h����AiO��A�t�y���gOS�: ��c��,ۈ����1VO���~�ś�u��W�Z��[��w`'}�"~qI��Q]�6ʏ�7�+ 1�U�k��_%E���&U�I]�;�~���B��8�J�+�w~W�b���.
Measuring the protein concentration of samples is essential for many protein assays, such as Western Blotting or Zymography. 2. Cite as. Figure 1.